Journal: Oxidative Medicine and Cellular Longevity

Article Title: Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis

doi: 10.1155/2022/2566917

Figure Lengend Snippet: PSP inhibited LPS-induced expression of calpain-1 and degradation of its substrates. (a–c) show the representative blots of calpain-1, calpain-2, calpastatin, and quantification data for calpain-1/ β -actin, calpain-2/ β -actin, and calpastatin/ β -actin in experiment 1. (d–f) show representative blots of calpain-1, calpain-2, calpastatin, and quantification data for calpain-1/ β -actin, calpain-2/ β -actin, and calpastatin/ β -actin. (g) shows representative blots of SCOP and PTEN. (h) shows the quantification data for SCOP/ β -actin and PTEN/ β -actin. Data are presented as the mean ± SEM. ∗ P < 0.05 vs. control, # P < 0.05 vs. LPS group.

Article Snippet: The membranes were blocked with 5% fat-free milk for 2 h at room temperature, then were incubated with primary antibodies against calpain-1 (1 : 1,000, ProteinTech), calpain-2 (1 : 1,000, ProteinTech), calpastatin (1 : 1,000, ProteinTech), suprachiasmatic nucleus circadian oscillatory protein (SCOP) (1 : 500, Santa Cruz), phosphatase and Tensin Homolog deleted on Chromosome 10 (PTEN) (1 : 1,000, CST), Nrf2 (1 : 1,000, ZEN BIO), heme oxygenase-1 (HO-1) (1 : 1,000, ZEN BIO), NLRP3 (1 : 1,000, CST), apoptosis-associated speck-like protein (ASC) (1 : 1,000, ZEN BIO), caspase-1 (1 : 1,000, ProteinTech), cleaved-caspase-1 (1 : 1,000, CST), glial fibrillary acidic protein (GFAP) (1 : 1,000, Bioss), extracellular signal-regulated kinase (ERK) (1 : 1,000, CST), p-ERK (1 : 1,000, CST), and nuclear factor-kappa B (NF- κ B) (1 : 1,000, CST) at 4°C overnight.

Techniques: Expressing, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis

doi: 10.1155/2022/2566917

Figure Lengend Snippet: NAC reduced oxidative stress levels and inhibited calpain-mediated inflammatory pathways. (a) shows the schematic design. (b, c) show SOD and MDA levels in the hippocampus. (d) shows representative blots of calpain-1, calpastatin, and PTEN. (e) shows quantification data for calpain-1/ β -actin, calpastatin/ β -actin, and PTEN/ β -actin in experiment 3. (f–i) show representative blots of p-ERK, NF- κ B, NLRP3, and IL-1 β and quantification data for p-ERK/ β -actin, NF- κ B/ β -actin, NLRP3/ β -actin, and IL-1 β / β -actin in experiment 3. Data are presented as the mean ± SEM. ∗ P < 0.05 vs. control, # P < 0.05 vs. LPS group.

Article Snippet: The membranes were blocked with 5% fat-free milk for 2 h at room temperature, then were incubated with primary antibodies against calpain-1 (1 : 1,000, ProteinTech), calpain-2 (1 : 1,000, ProteinTech), calpastatin (1 : 1,000, ProteinTech), suprachiasmatic nucleus circadian oscillatory protein (SCOP) (1 : 500, Santa Cruz), phosphatase and Tensin Homolog deleted on Chromosome 10 (PTEN) (1 : 1,000, CST), Nrf2 (1 : 1,000, ZEN BIO), heme oxygenase-1 (HO-1) (1 : 1,000, ZEN BIO), NLRP3 (1 : 1,000, CST), apoptosis-associated speck-like protein (ASC) (1 : 1,000, ZEN BIO), caspase-1 (1 : 1,000, ProteinTech), cleaved-caspase-1 (1 : 1,000, CST), glial fibrillary acidic protein (GFAP) (1 : 1,000, Bioss), extracellular signal-regulated kinase (ERK) (1 : 1,000, CST), p-ERK (1 : 1,000, CST), and nuclear factor-kappa B (NF- κ B) (1 : 1,000, CST) at 4°C overnight.

Techniques: Control